**Name: **Untitled

**Conformation: **linear

**Overhang: **five_prime, three_prime, blunt

**Minimum Site Length: **5 bases

**Maximum Number of Cuts: **all

**Included: **all commercial, prototypes only

**Noncutters: **AarI, AatII, AbsI, AccI, AclI, AcyI, AflII, AflIII, AgeI, AgsI, AjuI, AlfI, AloI, AlwNI, ApaI, ApaLI, ApoI, ArsI, AscI, AsuII, AvaI, AvaII, AvrII, BaeI, BalI, BamHI, BarI, BbvI, BbvCI, BcgI, BciVI, BclI, BdaI, BfiI, BglI, BglII, Bpu10I, BsaAI, BsaBI, BsaXI, BseMII, BsePI, BseRI, BseSI, BseYI, BsgI, BsmI, BsmAI, Bsp1407I, BspHI, BspMI, BsrI, BsrBI, BsrDI, BstEII, BstXI, BtgZI, BtrI, BtsI, CfrI, Cfr10I, ClaI, CspCI, DraII, DraIII, DrdI, Eam1105I, EciI, Eco31I, Eco47III, Eco57I, Eco57MI, EcoNI, EcoP15I, EcoRI, EcoRV, Esp3I, FalI, FauI, FokI, FseI, FspAI, GsuI, HaeII, HaeIV, HgaI, HindII, HindIII, HpaI, Hpy99I, KpnI, MauBI, MboII, MfeI, MluI, MmeI, MslI, NaeI, NarI, NcoI, NdeI, NheI, NmeAIII, NotI, NruI, NspI, OliI, PacI, PasI, PflMI, PfoI, PmaCI, PmeI, PpiI, PpuMI, PshAI, PsiI, PI-PspI, PspXI, PsrI, PstI, PvuI, PvuII, RsrII, SacI, SacII, SalI, SanDI, SapI, ScaI, PI-SceI, SduI, SexAI, SfaNI, SfiI, SgfI, SgrAI, SgrDI, SmaI, SmlI, SnaBI, SpeI, SphI, SrfI, Sse8387I, SspI, StuI, StyI, SwaI, TaqII, TatI, TauI, TfiI, TseI, TsoI, TspDTI, TspGWI, TspRI, TstI, Tth111I, XbaI, XcmI, XhoI, XhoII, XmnI

Name | Sequence | Site Length | Overhang | Frequency | Cut Positions |
---|---|---|---|---|---|

BccI | CCATC | 5 | five_prime | 1 | 53 |

EcoRII | CCWGG | 5 | five_prime | 1 | 79 |

PleI | GAGTC | 5 | five_prime | 1 | 71 |

Tsp45I | GTSAC | 5 | five_prime | 1 | 74 |

VspI | ATTAAT | 6 | five_prime | 1 | 15 |

HphI | GGTGA | 5 | three_prime | 1 | 68 |

BplI | GAGNNNNNCTC | 6 | three_prime | 2 | 54, 86 |

Hin4I | GAYNNNNNVTC | 6 | three_prime | 2 | 48, 80 |